Creative Proteomics Has Launched a Wide Range of Newly-developed Proteomics Analysis Services for Biologists

SHIRLEY, NY, US - Jun 25, 2019 - Creative Proteomics, a leading supplier in metabolomics services, has recently released its several newly-developed proteomics services to researchers. The service ranges include six: N-terminal sequence, protein quantification, TMT proteomics, SILAC proteomics analysis, and PTM analysis, which are based on some of the most powerful platforms and techniques in this field with unrivaled accuracy and sensitivity.

In protein N-terminal sequence analysis, the protein is first modified with phenyl isothiocyanate (PITC). Subsequently, the derivatized terminal amino acid is removed by acid cleavage in the form of a phenylhydantoin (PTH) derivative, and the new a-amino group on the next amino acid is now available for the next reaction with PITC. Therefore, the protein sequence is being analyzed by the severe reaction added by PITC, and one PTH is cut at a time for analysis. Assuming the reaction is completely effective, the method can sequence the entire amino acid from the N-terminus. Although less efficient than mass spectrometers, N-terminal Edman sequencing still has the advantage of protein analysis, while other analytical methods are not readily available. It is quite conventional to obtain sequences of at least 20 to 25 residues from the N-terminus of proteins and peptides using the prior art. Creative Proteomics provides N-terminal sequence analysis by Edman and mass spectrometry for therapeutic proteins, monoclonal antibodies and protein vaccines.

In order to assess the biological function of proteins and their modifications to understand the signaling mechanisms within cells and the specific biomarkers of disease, it is important to study changes in proteomes under different experimental conditions. Mass spectrometry-based quantitative proteomics emerging technologies provide powerful tools for quantitatively and systematically assessing quantitative differences in protein profiles across different samples and are increasingly becoming an important part of biomedical and clinical research. Based on protein quantification, the proteome of diseased samples is compared to normal proteomes worldwide and has been widely used to study various human diseases in order to identify biomarkers and/or to reveal the pathogenesis of the disease. .

Developed by Thermo Scientific, Tandem Mass Tag (TMT) is an in vitro labeling technique that allows simultaneous protein identification and relative quantification of different samples (up to 10) in the same experiment. In the TMT experiment, precursor ions were selected for fragmentation in full scan mode (MS1). And quantification was performed by measuring the intensity of the fragment reporter ions released from the label in tandem MS mode (MS2) during peptide fragmentation. Combined with ion trap and orbital trap technology, the reported signal can be measured after the third stage of ion isolation and fragmentation (MS3), which has been shown to greatly reduce the rate distortion caused by interfering ions. With years of experience, Creative Proteomics has developed an advanced platform with Q Exactive HF, Orbitrap Fusion and Orbitrap Fusion Lumos mass spectrometers for TMT proteomics analysis services. They offers professional all-inclusive services including sample preparation, digestion, TMT labeling, LC-MS / MS analysis and data analysis.

SILAC-based quantitative proteomics (SILAQ) is an innovative technique for high-throughput quantitative analysis of large protein complexes, protein-protein and protein-small molecule interactions. SILAC proteomics analysis service at Creative Proteomics provides an unbiased strategy that reveals the effects of inhibitors or other perturbations on protein dynamics and cell distribution. It can also be used as a sensitive and efficient method for determining specific interaction partners for proteins in cells.

Post-translational modification analysis (PTM) refers to the covalent, usually enzymatic, modification of proteins, as well as protein processes during or after protein biosynthesis. Protein post-translational modification (PTM) increases the functional diversity of the proteome by modifying functional proteins (such as phosphates, acetates, amide groups or methyl groups) and affects almost all of normal cell biology and pathogenesis aspects. It plays a key role in many cellular processes such as cell differentiation, protein degradation, signaling and regulation processes, gene expression regulation and protein-protein interactions. Modifications typically include phosphorylation, glycosylation, ubiquitination, nitrosylation, methylation, acetylation, lipidation, and proteolysis, and affect almost all aspects of normal cell biology and pathogenesis. Therefore, the characterization of PTM, including modification categories and modification sites, is critical for the study of cell biology and disease diagnosis and prevention.

“Creative Proteomics is a professional biotech company specializing in providing Proteomics services. State-of-the-art equipment, highly trained and experienced staff, as well as reference methodologies are utilized to enable us to deliver consistent and high-quality data with cost-efficiency. Based on highly experienced and knowledgeable in the application of wine analysis, Creative Proteomics can provide fast turnaround, clear concise written reports, and custom service to help customers resolve your analytical and technical challenges.” said Dr. Charlie.

About Creative Proteomics

Creative Proteomics, as an experienced proteomics, metabolomics, and bioinformatics services supplier, has been equipped with advanced technologies to meet the growing needs of researchers and scientists in proteomics and metabolomics related researches.

Contact:

Address: 45-1 Ramsey Road, Shirley, NY 11967, USA

Email: contact@creative-proteomics.com